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KMID : 0378019680110120031
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New Medical Journal
1968 Volume.11 No. 12 p.31 ~ p.42
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Immunologic Study on Human Chorionic Gonadotrophin
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Abstract
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For the practical use of immunological pregnancy test (11.1-R.), ´and technical familiarization, semi-quantitative determinations of H.C.G. were performed by means of hemagglutination inhibition technique.
1) H.C.G. of relatively high purity (5,427 IU/mg) was used as antigen and high titer of antiserum was obtained even with relatively small amount of antigen through booster injections of antigen and complete Freund´s adjuvant.
2) To prove the specificity of the obtained antiserum, weighing technique using i nmature rat ovary was applied, and it was found that the 0.1 ml of the obtained antiserum could neutralize the increasing effect of 200 IU of H.C.G. in immature ovarian weight.
3) Analysis of H.C.G. used in this work by Ouchterlony method showed only one clear precipitatin band between antigen and antiserum, indicating that used H.C.G. contained a negligible amount of other proteins with antigenicity.
4) The sensitivity of hemagglutination inhibition test in this study could detect 0.2 IU/ml of H.C.G.
5) The sensitivity of the hemagglutination inhibition reaction was so adjusted that more than 600 IU/1 of H. C. G. could be detectable, and urine to be examined was diluted I : 2 and I : 4 with the pH 6.4 phosphorus buffered saline. Therefore positive reactions obtained when the urine contained more than 1, 2002,400 IU/1 of H.C.G. and positive reactions were classified as pregnant cases. By doing so, the highest accuracy of the pregnancy test was obtained. A total of 523 cases, both pregnant and non-pregnant control, were analysed and it was disclosed that the over-all accuracy rate was 98.5 per cent for the 2 groups combined. It was particularly accurate in the non-pregnant control, the rate being 100 per cent, without a single case of false positive.
6) The´ urine of patients with cervical cancer or proteinuria was not caused false positive reaction in this study.
7) The semi-quantitative determination of H.C.G. in the random-urine of 340 pregnant women at various gestational period revealed that H.C.G. has been sharply increased after 5th week of gestation and reached to the peak in 9th10th week, giving an average of 118, 000 IU/1(4, 000512, 000 IU/1), then gradually declined to reach a point of stabilization in 24th week and 18,00026, 000 IU/1 has been maintained until the end of pregnancy.
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KEYWORD
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